Superoxide Dismutase 1 (SwissProt P00441) is a major antioxidant enzyme that catalyzes the conversion of superoxide anion to hydrogen peroxide and molecular oxygen. SOD1 is ubiquitously expressed and is localized in the cytosol, nucleus, and mitochondrial intermembrane space. Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1).
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:
ELISA: 0.03-1ug/ml
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.