SUPT5H, ID (SUPT5H, SPT5, SPT5H, Transcription elongation factor SPT5, DRB sensitivity-inducing factor 160kD subunit, DRB sensitivity-inducing factor large subunit, Tat-cotransactivator 1 protein) (APC)

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

042487-APC

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Allophycocyanin (APC).

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Wenzel, S., et al. Biochem. J. 425(2):373-380(2010)\nChen, Y., et al. Genes Dev. 23(23):2765-2777(2009)\nKomori, T., et al. Genes Cells 14(3):343-354(2009)\nChen, H., et al. PLoS ONE 4 (9), E6918 (2009) :\nAmir-Zilberstein, L., et al. J. Biol. Chem. 283(3):1317-1323(2008)

Additional Info

Human

Alternative Names

SUPT5H, SPT5, SPT5H, Transcription elongation factor SPT5, DRB sensitivity-inducing factor 160kD subunit, DRB sensitivity-inducing factor large subunit, Tat-cotransactivator 1 protein