Surfactant Protein A (SP-A)

Supplier:

United States Biological

Cat no: S8400-09

Surfactant Protein A (SP-A)

There are four main surfactant proteins, known as SP-A, B, C, and D. SP-A was the first surfactant protein to be identified, and is also the most abundant. Its molecular mass varies from 26-38kD. It can be found in an open or closed form depending on the other substances present in the system. Calcium ions produce the closed-bouquet form. SP-A is necessary for the production of tubular myelin, a lipid transport structure unique to the lungs.\n\nApplications: \nSuitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunohistochemistry: 1:200\nWestern Blot: 0.5ug/ml. Detected SP-A in 10ug of human lung tissue lysate, band at ~35kD.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb\nI1904-43: IgG, X-Adsorbed (Biotin) Pab Gt x Rb\nI1904-40E: IgG, H&L (Biotin) Pab Gt x Rb\nI1904-46P: IgG, H&L (Biotin) Pab Gt x Rb\nI1904-42T: IgG, H&L (Biotin) Pab Gt x Rb\nI1904-42V: IgG, H&L (Biotin) Pab Gt x Rb\n\nRecommended Control: \nHuman lung tissue lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S8400-09

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05mM sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1.

Additional Info

Recognizes the C-type lectin domain of human Surfactant Protein A. Species Crossreactivity: mouse.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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