Surfactant Protein B (SP-B)

There are four surfactant-specific proteins, designated surfactant protein A (SP-A), SP-B, SP-C and SP-D respectively. SP-A and SP-D are hydrophilic surfactant proteins and are members of the collectin family. SP-B and SP-C are hydrophobic surfactant proteins and may be the most appropriate indicators for the evolutionary origin of surfactant. SP-B is synthesized by the alveolar type II epithelial cells as a 40-42 kD precursor that is subsequently proteolytically processed to 7.8-8 kD. SP-B enhances the spreading and stability of surfactant phospholipids in the alveolus. SP-B is essential for air-breathing in mammals and is therefore largely conserved. SP-B can interact with both phospholipid head groups and fatty chains and is particularly active in enhancing surface active behavior in endogenous and exogenous lung surfactants. Even low SP-B contents had measurable effects in increasing the adsorption, dynamic surface tension lowering, and/or film respreading of DPPC, mixed synthetic lipids, and column-purified lung surfactant phospholipids. Deficiency of SP-B and other surfactant components is associated with respiratory distress syndrome (RDS) in premature infants and adults with respiratory distress syndrome (ARDS). \n\nApplications:\nSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:50. Recognizes proteins of 42, 25 and 18kD under non-reducing conditions. The 18kD form is reduced to 7.7-8kD in the presence of beta-mercaptoethanol.\nImmunohistochemistry (Frozen, paraffin): 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S8401-02D

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Bov, Prc

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Whitsett, J., et al., Immunologic identification of a pulmonary surfactant-assosiated protein of molecular weight = 6000 daltons. Pediatr. Res. 20: 744 (1986). 2. Weaver, T., et al., Identification of surfactant proteolipid SP-B in human surfactant and fetal lung. J. Appl. Physiol. 65: 982 (1988). 3. Gregory, T., el al., Surfactant chemical composition and biophysical activity in acute respiratory distress syndrome. J. Clin. Invest. 88: 1976 (1991). 4. Clark, J., et al., Targeted disruption of the surfactant protein B gene disrupts surfactant homeostasis, causing respiratory failure in newborn mice. PNAS 92: 7794 (1995). 5. Nogee, L., et al., Allelic heterogeneity in hereditary surfactant protein B (SP-B) deficiency. Am. J. Respir. Crit. Care Med. 161: 973 (2000).

Additional Info

Recognizes human Surfactant Protein B (SP-B). \nSpecies Crossreactivity: mouse, bovine and porcine