Survivin

Regulated inhibition of programmed cell death (apoptosis) preserves normal homeostasis and tissue and organ morphogenesis. Aberrations in this process contribute to human diseases and cancer by abnormally prolonging cell viability. Recently, several apoptosis inhibitors related to the baculovirus iap gene have been found in various species, including human. IAP proteins contain one/three Cys/His baculovirus IAP repeats plus a C-terminal RING finger and are thought to block an evolutionary conserved step in apoptosis. Survivin encodes a structurally unique inhibitor of apoptosis (IAP). Survivin expression is turned off during fetal development and is not found in non-neoplastic adult human tissues. Survivin becomes abundantly re-expressed in transformed cells and in all of the most common cancers of lung, colon, pancreas, breast and prostate in vivo. Survivin appears to be situated at the crossroads of cell death and cell division, governing a checkpoint involved in cytokinesis while also suppressing apoptosis. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury.\n\nApplications:\nSuitable for use in Flow Cytometry, Immunoprecipitation and Western Blot. Other applications not tested. \n\nRecommended Dilutions:\nFlow Cytometry: 1:200\nImmunoprecipitation: 1:100\nWestern Blot: 1:1000. Recognizes a band at ~16.5kD.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

S8500-03A

Size

100ul

Applications

FC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.6, 15mg/ml BSA, 0.05% sodium azide.

P Type

Mab

Purity

Ascities

Isotype

IgG2a,k

References

1. Fortugno, P., et al. J. of Cell Science. 2002; 115(3): 575-585. 2. Zhu, L., et al. FEBS Letters. 2001; 508: 369-374. 3. Allen, S., et al. Cancer Res. 2003; 63: 567-572. 4. Xu, Z., et al. Journal of Biological Chemistry. 2004; 279(3): 1838-1844. 5. Ryan, B., et al. Expression of survivin its splice variants survivin-2B survivin-delta Ex3 in breast cancer. British Journal of Cancer. 6. Ansell, S., et al. Leukemia. 2004; 18: 616-623. 7. Brocardo, M., Y. Lei, et al. J. Biol. Chem. 2008; 283: 5950-5959. 8. Hino R, Uozaki H, Inoue Y, et al. Cancer Res. March 1, 2008;68(5):1427-35. 9. Levkau B, Schafers M, Wohlschlaeger J, et al. Circulation. March 25, 2008;117(12):1583-93. 10. Huang H, Fletcher L, Beeharry N, et al. Cancer Res. May 15, 2008;68(10):3724-32.

Additional Info

Recognizes the cytoplasmic form of human survivin. Species Crossreactivity: mouse and rat.