Synapsin I, phosphorylated (Ser549) (Synapsin 1, Synapsin-1, SYN1, SYN-1, Synapsin-I, SYNI, SYN-I, Brain Protein 4.1, SYN1a, SYN1b, SYN-1a, SYN-1b)

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Ser 549 along with Ser 62 and Ser 67 are the sites of Synapsin I that are phosphorylated by MAP kinase (Jovanovic et al., 1996). Phosphorylation and subsequent dephosphorylation of this site is thought to play a key role in synaptic vesicle trafficking.\n\nApplications: \nSuitable for use in Immunohistochemistry, Dot Blot and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: paraffin sections\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

S9103-90B

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can

Form

Supplied as a liquid in 10mM Hepes, pH 7.5, 150mM sodium chloride, 10mg/ml BSA, 50% glycerol

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Jovanovic JN, et al., (1996) Neurotrophins stimulate phosphorylation of Synapsin I by MAP kinase and regulate SynapsinI-actin interactions. Neurobiology 93:3679-3683.

Additional Info

Recognizes rat synapsin I doublet phosphorylated at Ser549. MW: 78kD. Immunolabeling of the Synapsin I band is blocked by lambda-phosphatase treatment. Species sequence homology: human, mouse, primate, bovine and canine.