Synapsin I, phosphorylated, Ser553

Synapsin I is a member of neuron-specific phosphoproteins that associate with synaptic vesicles (1). Three mammalians synapsins have been identified, Synapsin I, II and III. Generated by differential splicing, Synapsin Ia and Synapsin Ib differ in their Carboxyl termini (2). Synapsin I also functions in binding synaptic vesicles to several elements of the cytoskeleton including actin filaments, microtubules and spectrin. (3) Fairly abundant protein of the brain (0.4%), Synapsin I is best known to be CaM kinase II substrate in addition to cAMP-dependent protein kinase. (4) Phosphorylation at site Ser 566 and Ser 603 by CaM kinase II will cause a 5 fold decrease in the affinity of synaptic vesicles binding (5).\n\nApplications: \nSuitable for use in Western Blotting, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:10,000-50,000 (74kD)\nImmunocytochemistry: 1:50-100\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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