Synapsin I (Synapsin 1, Synapsin-1, SYN1, SYN-1, Synapsin-I, SYNI, SYN-I, Brain Protein 4.1, SYN1a, SYN1b, SYN-1a, SYN-1b)

Synapsin I plays a key role in synaptic plasticity in the brain. This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation. Thus Synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S9104-01G

Size

50ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

10 mM Hepes (pH7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol

P Type

Pab

Purity

Serum

References

1. Feng J, Chi P, Blanpied TA, Xu YM, Magarinos AM, Ferreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA, Augustine GJ, Greengard P (2002) Regulation of neurotransmitter release by synapsin III. J Neurosci 22:4372- 4380.\n\n2. Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC, Jr., Greengard P, Czernik AJ (2001) Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+- ependent glutamate release in isolated nerve terminals. J Neurosci 21:7944-7953.\n\n3. Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P (2002) A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neurosci 5:431-437.\n\n4. Moore RY, Bernstein M (1989) Synaptogenesis in the rat suprachiasmatic nucleus demonstrated by electron microscopy and synapsin I immunoreactivity. J Neurosci 9:2151-2162.\n\n5. Nayak AS, Moore CI, Browning MD (1996) CaM kinase II phosphorylation of the presynaptic protein synapsin is persistently increased during expression of long-term potentiation. Proc Natl Acad Sci (USA) 93:15451-15456. \n\n6. Stone LM, Browning MD, Finger TE (1994) Differential distribution of the synapsins in the rat olfactory bulb. J Neurosci 14:301-309.

Additional Info

Specific for the ~78k Synapsin I protein doublet. Immunolabeling blocked by preadsorption of the antibody with the protein used to generate the antibody. Species Crossreactivity: Human, rat, and mouse.