Synaptophysin is a protein of the presynaptic vesicle exocytosis machinery interacting at the vesicle with VAMP/synaptobrevin. Synaptophysin structure consists of a hexameric protein of four transmembrane spans with cytoplasmic amino and carboxyl termini. Although its actual function remains elusive, synaptophysin remains a specific presynaptic marker for all neurons, neuroendocrine cells and neuroderived neoplasms.
Applications:
Suitable for use in Western Blot, Immunohistochemistry, and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:10000
Immunohistochemistry: 1:250-1:500
Immunocytochemistry: 1:250
Optimal dilutions to be determined by the researcher.
Positive Control:
PC12 cell lysate
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.