Synaptotagmin, phosphorylated (Ser309) (SYT, DKFZp781D2042, P65, SVP65, Synaptotagmin I, SYT1, SYT-1, SytI, SYT2, SYT3)

Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).\n\nApplications: \nSuitable for use in Dot Blot and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S9108-99B1

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg per ml BSA and 50% glycerol

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Poskanzer, K.E., Marek, K.W., Sweeney, S.T. and Davis, G.W., \"Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo,\" Nature (London) 426 (2003) 559 - 563.\n2. Wang, C.T., Lu, J.C., Bai, J.H., Chang, P.Y., Martin, T.F.J., Chapman, E.R. and Jackson, M.B. \"Different domains of synaptotagmin control the choice between kiss-and-run and full fusion,\" Nature (London) 424 (2003) 943 - 947.\n3. Fernandez-Chacon, R., Konigstorfer, A., Gerber, S.H., Garcia, J., Matos, M.F., Stevens, C.F., Brose, N., Rizo, J., Rosenmund, C. and Sudhof, T.C., \"Synaptotagmin I functions as a calcium regulator of release probability,\" Nature (London ) 410 (2001) 41 - 49.\n4. Hilfiker, S., Pieribone, V.A., Nordstedt, C., Greengard, P., and Czernik, A.J., \"Regulation of synaptotagmin I phosphorylation by multiple protein kinases,\" J. Neurochem. 73 (1999) 921 - 932.\n5. Lee BH, Min X, Heise CJ, Xu BE, Chen S, Shu H, Luby-Phelps K, Goldsmith EJ, Cobb MH (2004) WNK1 phosphorylates synaptotagmin 2 and modulates its membrane binding. Mol Cell 15:741-751.

Additional Info

Specific for the ~60 - 62k Synaptotagmin protein phosphorylated at Ser309 in Western Blots of rat brain lysates. Species Crossreactivity: This antibody recognizes the rat form of the protein. \nOther species have not been tested. Human, mouse, and rat have 100% amino acid sequence identity with the antigen used to raise the antibody.