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Syndecan-1 (CD138, SDC, SYND1, Syndecan)

Cat no: S9111-30B

Syndecan-1 (CD138, SDC, SYND1, Syndecan)

Syndecan-1 is a transmembrane (Type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization. Bearing properties of both heparan sulfate and chondroitin sulfate, Syndecan-1 functions as a cell surface proteoglycan membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered Syndecan-1 expression has been detected in several different tumor types.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000 using mouse liver lysate. Proteins were visualized using a IgG (HRP) Dk x Rb and chemiluminescence detection. A dimer of Syndecan-1 was identified at ~77kD while monomeric Syndecan-1 is visible at ~32kD. The mature form, with heparan and chondroitin sulfate addtions, can sometimes be seen between 500-1000kD but may need to be solubilized from whole tissues by urea extraction. Triton X-100 or other mild detergents are enough to release the immature precursor forms.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies: \nI1904-46E: IgG, H&L (HRP) Pab Dk x Rb\nI1904-46K: IgG, H&L, X-Adsorbed (HRP) Pab Dk x Rb\nI1903-89G5: IgG, H&L, X-Adsorbed (HRP) Pab Dk x Rb\nI1904-45P6: IgG, H&L (HRP) Pab Dk x Rb\n\nPositive Control: \nMouse liver lysate \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S9111-30B

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Wilsie, L.C., et al., Lipids Health Dis. 5: 23 (2006).

Additional Info

Recognizes mouse Syndecan-1 protein.

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