Syndecan 1

Syndecans 1-4 are heparan sulfate proteoglycans (HSPGs), which are cell-surface-associated proteins. Syndecans contain a small core protein with distinct functional domains to which are linked multiple glycosaminoglycan (GAG) chains, predominantly of the heparan sulfate variety. Syndecan synthesis is highly regulated and is dependent on both cell type and developmental state. Syndecans are involved in cell adhesion, tissue morphogenesis, and differentiation and regulation of cell responsiveness to soluble growth-regulatory compounds. These functions are attributed primarily to the glycan moieties. Syndecans also interact with growth factors such as fibroblast growth factor and epidermal growth factor. \n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nELISA (Immunogen): 0.1-1ug/ml \nWestern Blot (Human): 1-3ug/ml; Confirmed with human T47D, SW-480 and PC-3 cell lysates. A single band at ~90kD representing the glycosylated form of Syndecan 1 is observed and occasionally a band of ~31kD representing the unglycosylated form of Syndecan 1 may be observed. \nImmunohistochemistry (FFPE): 10ug/ml; For best results heat induced epitope retrieval (HIER) with EDTA, pH 8.0, is required prior to staining. Confirmed with human gastric cancer and breast cancer tissues, as well as with mouse and rat stomach, small bowel and skin tissues. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S9111-40A

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Elenius, K., et al., J. Cell Biol. 114: 585-595 (1991). 2. Bayer-Garner, et al., J. Cutan. Pathol. 28: 135-139 (2001). 3. Conejo, J.R., et al., Int. J. Cancer 88: 12-20 (2000). 4. Kim, C.W., et al., Mol. Biol. Cell 5: 797-805 (1994). 5. Derksen, P.W., et al., Blood 99: 1405-1410 (2002). 6. Dhodapkar, M.V., et al., Br. J. Haematol. 99: 368-371 (1997). 7. Liebersbach, B.F., et al., J. Biol. Chem. 269: 20,013-20,019 (1994). 8. Matsuda, K., et al., Cancer Res. 61: 5562-5569 (2001). 9. Mukunyadzi, P., et al., Mod. Pathol. 15: 45-49 (2002). 10. Subramanian, S.V., et al., J. Biol. Chem. 272: 14,713-14,720 (1997). 11. Yeaman, C. & Rapraeger, A.C., J. Cell Biol. 122: 941-950 (1993). 12. Mundhenke, C., et al., Am. J. Pathol. 160: 185-194 (2002). 13. Ridley, R.C., et al., Blood 81: 767-774 (1993). 14. Seidel, C., et al., Blood 96: 3139-3146 (2000). 15. Stanley, M.J., et al., J. Biol. Chem. 270: 5077-5083 (1995).

Additional Info

Recognizes human Syndecan 1. \nSpecies Crossreactivity (IHC): mouse and rat.