Syndecan 2 (APC)

Syndecan 2 is a member of the syndecan family of Type 1 trans- membrane proteins capable of carrying heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans. The four vertebrate syndecans show conserved cytoplasmic domains and divergent extracellular portions (except for GAG attachment sites). Among the Syndecans, Syndecan 2 is most similar to Syndecan 4. Human Syndecan 2 is synthesized as 201aa core protein with an 18aa signal sequence, a 126aa extracellular domain (ECD), a 25aa transmembrane region and a 32aa cytoplasmic tail. The human ECD of Syndecan 2 contains three closely-spaced consensus Ser-Gly sequences for the attachment of HS side chains. It shares 76%, 73%, 87%, 78% and 63% aa identity with the ECD of mouse, rat, bovine, dog and chicken Syndecan 2, respectively. The cytoplasmic tail has both serine and tyrosine phosphorylation sites. Addition of 20-80 disaccharides per side chain adds considerably to the size of the 22kD core protein. Non-covalent homodimerization of Syndecan 2 is dependent on the transmembrane domain. Syndecan 2 is expressed in cells of mesenchymal origin, neuronal and epithelial cells, and is the predominant syndecan expressed during embryonic development. Expression is upregulated in several cancer cell lines. After induction in macrophages by inflammatory mediators, Syndecan 2 selectively binds FGF basic, VEGF and EGF. Syndecan 2 expressed on human primary osteoblasts binds GM-CSF and may function as a co-receptor. Activated endothelial cell Syndecan 2 specifically binds IL-8 and may participate in promoting neutrophil extravasation by forming a chemotactic IL-8 gradient. Typically, cytokine, chemokine and extracellular matrix protein binding occurs through interaction with HS side chains, but the Syndecan 2 extracellular domain can bind TGF-beta directly via protein-protein interaction.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested. \n\nRecommended Dilutions:\nFlow Cytometry: 10ul labels 1x10e5 cells or 50ul packed whole blood\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C. Stable for 12 months at 4 degrees C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyaninconjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

S9111-40B2

Size

100Tests

Applications

FC

Hosts

Rat

Reactivities

Hum

Form

Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Tkachenko, E. et al. (2005) Circ. Res. 96:488. 2. Oh, E-S. and J.R. Couchman (2004) Mol. Cells 17:181. 3. Essner, J.J. et al. (2006) Int. J. Biochem. Cell Biol. 38:152. 4. Marynen, P. et al. (1989) J. Biol. Chem. 264:7017. 5. Choi, S. et al. (2005) J. Biol. Chem. 280:42573. 6. Park, H. et al. (2002) J. Biol. Chem. 277:29730. 7. Clasper, S. et al. (1999) J. Biol. Chem. 274:24113. 8. Modrowski, D. et al. (2000) J. Biol. Chem. 275:9178. 9. Halden, Y. et al. (2004) Biochem. J. 377:533. 10. Chen, L. et al. (2004) J. Biol. Chem. 279:15715.

Additional Info

Recognizes human Syndecan 2.