Syndecan 4 (Syndecan 4 (Amphiglycan, Ryudocan), Syndecan-4, Syndecan Proteoglycan 4, SDC4, SYND4, Amphiglycan, Ryudocan Core Protein, MGC22217, OTTHUMP00000031788, Ryudocan Amphiglycan, Ryudocan)

Syndecans are heparan sulfate proteoglycans (HSPGs), which are cell-surface-associated proteins. These include Syndecans. Syndecans contain a small core protein with distinct functional domains to which are linked multiple glycosaminoglycan (GAG) chains, predominantly of the heparan sulfate variety. Syndecan synthesis is highly regulated and is dependent on both cell type and developmental state. Syndecans are involved in cell adhesion, tissue morphogenesis and differentiation and regulation of cell responsiveness to soluble growth-regulatory compounds. These functions are attributed primarily to the glycan moieties. Syndecans also interact with growth factors such as fibroblast growth factor and epidermal growth factor.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nELISA (Immunogen): 0.1-1ug/ml. \nWestern Blot: 1-3ug/ml. \nOptimal dilutions to be determined by the researcher.\n\nPositive Controls: \nHuman T47D, HEP-G2, NCI-H69, SCLC and JEG cells lysates.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S9111-40C

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Elenius, K., et al. J Cell Biol 114, 585-95, 1991. 2. Yeaman, C. et al. J Cell Biol 122, 941-50, 1993. 3. Subramanian S. V., et al. J Biol Chem 272, 14713-20, 1997. 4. Kim C. W., et al. Mol Biol Cell 5, 797-805, 1994.

Additional Info

Recognizes human Syndecan 4 at ~37kD, representing the core protein, and a band of ~22kD, representing the unglycosylated form.