SynGAP (Synaptic Ras GTPase Activating Protein, Synaptic Ras GTPase-activating Protein 1, Synaptic Ras-GAP 1, SYNGAP1, KIAA1938, MRD5, Neuronal RasGAP, Ras GTPase-activating Protein SynGAP, RASA1, RASA5)

SynGAP is a synaptic GTPase-activating protein selectively expressed in the brain and found at\nhigher concentrations specifically at excitatory synapses in the mammalian forebrain. SynGAP has a PH domain, a C2 domain, and a highly conserved RasGAP domain, which negatively regulates both Ras activity and its downstream signaling pathways. SynGAP interacts with the PDZ\ndomains for SAP102, as well as PSD95, a postsynaptic scaffolding protein that couples SynGAP to NMDA receptors (1). SynGAP is phosphorylated by Ca2+/calmodulin-dependent protein kinase II (CaMKII) at Ser765 and Ser1123 among other sites (2,3). Phosphorylation of SynGAP\nresults in stimulation of GTPase activity of Ras, and PSD95 dependent CaMKII phosphorylation of SynGAP increases after transient brain ischemia (1,4). SynGAP is implicated in NMDAR- and CaMKII-dependent regulation of AMPAR trafficking and plays an important role in synaptic plasticity (3,5). SynGAP is critical during neuronal development since mice lacking SynGAP protein die postnatally. Furthermore, SynGAP mutant mice have reduced LTP and perform poorly\nin spatial memory tasks (6).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S9115-04C

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified

Isotype

IgG

References

(1) Kim, J.H. et al. (1998) Neuron 20, 683-91. (2) Oh, J.S. et al. (2004) J Biol Chem 279, 17980-8.\n(3) Krapivinsky, G. et al. (2004) Neuron 43, 563-74. (4) Song, B. et al. (2004) Brain Res 1005, 44-50.\n(5) Komiyama, N.H. et al. (2002) J Neurosci 22, 9721-32. (6) Kim, J.H. et al. (2003) J Neurosci 23, 1119-24.

Additional Info

Recognizes endogenous levels of total SynGAP. Species Crossreactivity: mouse, rat.

Alternative Names

DKFZp761G1421