Home  >  Products  >  SynGAP (Synaptic Ras GTPase Activating Protein, Synaptic Ras GTPase-activating Protein 1, Synaptic Ras-GAP 1, SYNGAP1, KIAA1938, MRD5, Neuronal RasGAP, Ras GTPase-activating Protein SynGAP, RASA1, RASA5)
SynGAP (Synaptic Ras GTPase Activating Protein, Synaptic Ras GTPase-activating Protein 1, Synaptic Ras-GAP 1, SYNGAP1, KIAA1938, MRD5, Neuronal RasGAP, Ras GTPase-activating Protein SynGAP, RASA1, RASA5)

SynGAP (Synaptic Ras GTPase Activating Protein, Synaptic Ras GTPase-activating Protein 1, Synaptic Ras-GAP 1, SYNGAP1, KIAA1938, MRD5, Neuronal RasGAP, Ras GTPase-activating Protein SynGAP, RASA1, RASA5)

Cat no: S9115-04C

Supplier: United States Biological
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SynGAP is a synaptic GTPase-activating protein selectively expressed in the brain and found at higher concentrations specifically at excitatory synapses in the mammalian forebrain. SynGAP has a PH domain, a C2 domain, and a highly conserved RasGAP domain, which negatively regulates both Ras activity and its downstream signaling pathways. SynGAP interacts with the PDZ domains for SAP102, as well as PSD95, a postsynaptic scaffolding protein that couples SynGAP to NMDA receptors (1). SynGAP is phosphorylated by Ca2+/calmodulin-dependent protein kinase II (CaMKII) at Ser765 and Ser1123 among other sites (2,3). Phosphorylation of SynGAP results in stimulation of GTPase activity of Ras, and PSD95 dependent CaMKII phosphorylation of SynGAP increases after transient brain ischemia (1,4). SynGAP is implicated in NMDAR- and CaMKII-dependent regulation of AMPAR trafficking and plays an important role in synaptic plasticity (3,5). SynGAP is critical during neuronal development since mice lacking SynGAP protein die postnatally. Furthermore, SynGAP mutant mice have reduced LTP and perform poorly in spatial memory tasks (6). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S9115-04C
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Purified
Alternative names: DKFZp761G1421
References: (1) Kim, J.H. et al. (1998) Neuron 20, 683-91. (2) Oh, J.S. et al. (2004) J Biol Chem 279, 17980-8. (3) Krapivinsky, G. et al. (2004) Neuron 43, 563-74. (4) Song, B. et al. (2004) Brain Res 1005, 44-50. (5) Komiyama, N.H. et al. (2002) J Neurosci 22, 9721-32. (6) Kim, J.H. et al. (2003) J Neurosci 23, 1119-24.
Additional info: Recognizes endogenous levels of total SynGAP. Species Crossreactivity: mouse, rat.

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