Synip (Syntaxin 4-Interacting protein)

Synip (Syntaxin 4-Interacting protein) specifically binds to Syntaxin 4 and is uniquely expressed in muscle and adipose tissue. The binding interaction of Synip with syntaxin 4 is regulated by insulin, which also induces dissociation of the Synip-syntaxin 4 complex. Synip plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane and functions as a positive regulator of insulin-stimulated GLUT4 translocation. Synip also competes with VAMP2 but Not SNAP23 for binding to Syntaxin 4 (1-3).\n\nApplications: \nSuitable for use in Flow Cytometry, Western Blotting, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:5,000-20,000 (70kD)\nImmunohistochemistry: 1:250-500\nFlow Cytometry: 1:40\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

S9115-25

Size

100ul

Applications

FC, IHC, WB

Hosts

Rabbit

Form

Supplied as a liquid.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Jeffrey E. Pessin, Debbie C. Thurmond, Jeffrey S. Elmendorf, Kenneth J. Coker, and Shuichi Okada, THE JOURNAL OF BIOLOGICAL CHEMISTRY. Vol. 274, No. 5, Issue of January 29, pp. 2593

Additional Info

Recognizes human Synip. Predicted to detect the isoform 2 base on sequence analysis.