Syntaxin 2 (Epimorphin)

Syntaxin 2, also known as epimorphin, is a 35kD type II integral membrane that belongs to the t-SNARE family, a group of proteins involved in protein transport (2). Syntaxin 2 was first identified as a factor that promotes branching morphogenesis in mammary epithelial cells (3). Studies using pancreatic exocrine acinar cells, in which the exocytic pathways are well defined, show primary localization of syntaxin 2 to the apical plasma membrane (4). Homologs of syntaxin 2 have been identified in various eukaryotes ranging from yeast to human (5, 6). Neuronal syntaxin is a component of a 20S complex implicated in vesicle docking and fusion, which involves its interaction with syntaxin through a ATP-dependent reaction modulated by synaptotagmin, SNAP-25, alpha-SNAP, and NSF (7). \n \nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nPositive Controls:\nJurkat Cell Lysate\nHeLa Cell Lysate \nRat Brain Tissue Extract\nMouse Brain Tissue Extract\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S9205-06

Size

100ug

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, Dro/Arth, NHP, Prc, Rabbit, Sheep, Xen/Amph

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. GenBank Accession Number L20823. 2. Bennett, M., Garcia-Arraras, J.E., Elferink, L.A., Peterson, K., Fleming, A.M., Hazuka, C.D. and Scheller, R.H. (1993) Cell 74: 863-873. 3. Hirai, Y., Lochter, A., Galosy, S., Koshida, S., Niwa, S. and Bissell, M.J. (1998) J. Biol. Chem. 140: 159-169. 4. Gaisano, H.Y., Ghai, M., Malkus, P.N., Sheu, L., Bouquillon, A., Bennett, M.K. and Trimble, W.S. (1996) Mol. Biol. Cell 12: 2019-2027. 5. Sollner, T., Whiteheart, S.W., Brunner, M., Erdjument-Bromage, H., Geromanos, S., Tempst, P. and Rothman, J.E. (1993) Nature 362: 318-324. 6. Calakos, N., and Scheller, R. (1996) Physiological Reviews 76: 1-29. 7. Sollner, T., Bennett, M.K., Whiteheart, S.W., Scheller, R.H., & J.E. Rothman (1993) Cell 75: 409-418. 8. Karim, S., Essenberg, R.C., Dillwith, J.W., Tucker, J.S., Bowman, A.S., Sauer, J.R. (2002) Insect Biochem Mol Biol 32(12): 1711-1721.

Additional Info

Recognizes rat Syntaxin 2 at ~35kD. Species Crossreactivity: human, mouse, bovine, canine, chicken, Drosophila, hamster, monkey, porcine, rabbit, sheep and Xenopus.