T-complex Protein 1 beta (T-complex Protein 1 beta Subunit, TCP1 beta, TCP-1 beta, Chaperonin Containing T-complex Polypeptide 1 beta Subunit, CCTB, Chaperonin Containing TCP1 Subunit 2 (beta), CCT2)

Applications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: Cytoplasmic\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T2035-10B

Size

50ug

Applications

WB

Hosts

Rat

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, NHP, Prc, Rabbit, Sheep

Form

PBS, 50mM Sodium Chloride, 10mM Sodium Phosphate, 0.1mM PMSF. pH 7.2

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG2b

References

Hynes G et al. Antibody characterisation of two distinct conformations of the chaperonin-containing TCP-1 from mouse testis. FEBS Lett 358:129-32 (1995). <PUBMED:7828721>Kubota H et al. Identification of six Tcp-1-related genes encoding divergent subunits of the TCP-1-containing chaperonin. Curr Biol 4:89-99 (1994). <PUBMED:7953530>Willison K et al. The t complex polypeptide 1 (TCP-1) is associated with the cytoplasmic aspect of Golgi membranes. Cell 57:621-32 (1989). <PUBMED:2655925>Willison KR et al. Molecular cloning and sequence analysis of a haploid expressed gene encoding t complex polypeptide 1. Cell 44:727-38 (1986). <PUBMED:3753900>

Additional Info

This antibody does not react with human Hsp60 nor with E. coli GroEL proteins. Species Crossreactivity: Crossreacts with Human, Mouse, Rat, bovine, Chicken, canine, Hamster, Monkey, porcine, Rabbit and Sheep.