TAF2, CT (TAF2, CIF150, TAF2B, Transcription initiation factor TFIID subunit 2, 150kD cofactor of initiator function, RNA polymerase II TBP-associated factor subunit B, TBP-associated factor 150kD, Transcription initiation factor TFIID 150kD subunit) (Azi

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\nImmunohistochemistry: 1:50-100\nFlow Cytometry: 1:10-50\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze horseradish peroxidase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

042579-HRP

Size

200ul

Applications

ELISA, FC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Conjugated to HRP.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Olsen, J.V., et al. Cell 127(3):635-648(2006)\nKim, J.E., et al. J. Proteome Res. 4(4):1339-1346(2005)\nGuermah, M., et al. Mol. Cell 12(4):991-1001(2003)

Additional Info

Human, mouse

Alternative Names

TAF2, CIF150, TAF2B, Transcription initiation factor TFIID subunit 2, 150kD cofactor of initiator function, RNA polymerase II TBP-associated factor subunit B, TBP-associated factor 150kD, Transcription initiation factor TFIID 150kD subunit