TAFA1 (FAM19A1, Protein FAM19A1, Chemokine-like Protein TAFA-1)

TAFA1 is a secreted, 13kD member of the FAM19/TAFA family of chemokine-like proteins. It is synthesized as a 133aa precursor that contains a 19aa signal sequence and a 114aa mature chain. Like other members of the FAM19/TAFA family, mature TAFA1 contains 10 regularly spaced cysteine residues that follow the pattern CX7CCX13CXCX14CX11CX4CX5CX10C, in which C represents a conserved cysteine residue andxrepresents a noncysteine aa. Human TAFA1 is 100% aa identical to mouse TAFA1. TAFA1 is expressed exclusively in the brain, with highest expression in the frontal cortex, temporal cortex, occipital cortex, parietal cortex and medulla, and low levels in the basal ganglion, thalamus, and cerebellum. The biological functions of TAFA family members remain to be determined, but there are a few tentative hypotheses. First, TAFAs may modulate immune responses in the CNS by functioning as brain-specific chemokines, and may act with other chemokines to optimize the recruitment and activity of immune cells in the CNS. Second, TAFAs may represent a novel class of neurokines that act as regulators of immune nervous cells. And third, TAFAs may control axonal sprouting following brain injury.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and Neutralization. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1ug/ml\nImmunohistochemistry: 5-15ug/ml\nNeutralization: 15ug/ml for 10ug/ml recombinant human TAFA-1.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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