logo
logo
Talin (KIAA1027, TLN, TLN1, ILWEQ)

Cat no: T0750

Talin (KIAA1027, TLN, TLN1, ILWEQ)

Applications: \nSuitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml detects Talin in a human A431 RIPA cell lysate. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with T0750 (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.\n\nImmunocytochemistry/Immunohistochemistry: 10ug/ml of a previous lot showed positive immunostaining for Talin in Ref-52 cells fixed with 4% paraformaldhyde and permea-bilized with 0.25% Triton X-100. A previous lot of this antibody detected talin in frozen sections.\n\nImmunoprecipitation: 4ug detects Talin from a human A431 RIPA lysates.\n\nPositive Control Included:\nA0001-50: Non-stimulated A431 cell lysate. Use 20ug per lane for minigels. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T0750

Size

200ug

Applications

ICC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Ch/Bird, Rabbit

Form

Supplied as liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Ion-exchange chromatography.

Isotype

IgG1

References

General Reference: Goldmann, W.H. and G. Isenberg, Biochem. Soc. Trans. 20: 2735, 1992.

Additional Info

Recognizes human Talin at ~ 200kD. Species crossreactivity: Rabbit and chicken. Does not cross-react with mouse or rat.

Read more on Supplier website

Latest promotions

Spend less time on DNA cleanup so you can do more science. The MSB Spin PCRapace is the fastest way to purify your DNA from PCR, restriction digestion, and...

Genovis introduces GlycINATOR™• Works on most IgGs• Effective on high-mannose and bisected N-linked Fc-glycans • Rapid – 30-minute...

Use promo code EASY3 to receive 13% off and FREE shipping!Experience a new dimension of electronic pipetting with the NEW Easypet 3 pipette controller. The...

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon™ BV421 and...

As an incentive to qualify our BSA, we are offering a 20% discount when you purchase your first 100g, 500g or 1000g of any grade of Bovine Serum Albumin....

It is not every day that you are given something for nothing. We are giving away additional spectrophotometer software.Cecil Instruments have enhanced the...

We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!

For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...

10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...

Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...

Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...

Jenway’s 73 series spectrophotometer range provides four models with a narrow spectral bandwidth of 5nm and an absorbance range of –0.3 to 2.5A,...

Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...