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Tau, aa1-352, Recombinant, Human (Microtubule-associated protein tau)

Cat no: T1035

Tau, aa1-352, Recombinant, Human (Microtubule-associated protein tau)

MAPT, also known as TAU, is a neuronal microtubule associated protein found predominantly on axons. The function of this protein is to promote tubulin polymerisation and stabilise microtubules, but it also serves to link certain signalling pathways to the cytoskeleton. MAPT, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer's disease (AD) brain.\n\nSource:\nRecombinant human MAPT protein, fused to His-tag at N-terminus, expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKAEEAGI GDTPSLEDEA AGHVTQARMV \nSKSKDGTGSD DKKAKGADGK TKIATPRGAA PPGQKGQANA TRIPAKTPPA PKTPPSSGEP PKSGDRSGYS SPGSPGTPGS RSRTPSLPTP \nPTREPKKVAV VRTPPKSPSS AKSRLQTAPV PMPDLKNVKS KIGSTENLKH QPGGGKVQIV YKPVDLSKVT SKCGSLGNIH HKPGGGQVEV \nKSEKLDFKDR VQSKIGSLDN ITHVPGGGNK KIETHKLTFR ENAKAKTDHG AEIVYKSPVV SGDTSPRHLS NVSSTGSIDM VDSPQLATLA \nDEVSASLAKQ GL\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T1035

Size

50ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.2M sodium chloride, 1mM DTT, 10% glycerol.

Purity

~85% (SDS-PAGE)

References

1. Jack CR Jr., et al. (2010) Lancet Neurol. 9(1):119-28. 2. Buescher JL., et al. (2010) J Biol Chem. 285(11):7957-63.

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Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can, NHP, Prc, Xen/Amph, ZF/Fish

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

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Applications

WB

Hosts

Mouse

Reactivities

Hum

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Applications

ICC, WB

Hosts

Mouse

Reactivities

Hum

More info
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