TCF-1 (F26) pAb

T-cell factor-1 (TCF-1) is a DNA-binding transcriptional activator that isessential for lymphoid cell development. The TCF family of transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. Several alternative splice variants of TCF-1 have been identified, including TCF-1A, which share a conserved amino terminus and differ in the carboxy terminal sequences. The Wnt mediated signaling pathway induces cytosolic (beta)-catenin binding to TCF proteins within the nucleus, leading to the enhanced expression of the Wnt target genes. The (beta)-catenin-TCF complexes are negatively regulated by the adenomatous polyposis coli (APC) tumor suppressor protein, which phosphorylates (beta)-catenin and, in turn, increases the degradation of cytosolic (beta)-catenin and inhibits the transcriptional activity of the TCF proteins.

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SPECIFICATIONS

Catalog Number

BS1987

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids N-terminus of Human TCF-1.

Species

TCF-1 (F26) pAb detects endogenous levels of TCF-1 protein.

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200

Swiss Prot

P36402

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul