TCF-3 (N601) pAb

Heterodimers between TCF3 and tissue specific basic helix loop helix (bHLH) proteins play major roles in determining tissue specific cell fate during embryogenesis, like muscle or early B cell differentiation. Dimers bind DNA on E box motifs: 5' CANNTG 3'. Binds to the kappa E2 site in the kappa immunoglobulin gene enhancer.

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SPECIFICATIONS

Catalog Number

BS2466

Size

100ug/100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 570-620 of Human TCF-3.

Species

TCF-3 (N601) pAb detects endogenous levels of TCF-3 protein.

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200

Swiss Prot

P15923

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 60 kDa

Alternative Names

Transcription factor E2-alpha; Class B basic helix-loop-helix protein 21; bHLHb21; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; Kappa-E2-binding factor; Transcription factor 3; TCF-3; Transcription factor ITF-1; TCF3; BHLHB21; E2A; ITF1