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TFIIA-(alpha) (E311) pAb

TFIIA-(alpha) (E311) pAb

Cat no: BS2282

Supplier: Bioworld Technology, Inc.
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Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. Recognition of the TATA binding element by TBP, one of the first steps in transcription initiation, may be regulated by TFIIA. TFIIA consists of three subunits designated TFIIA-(alpha), TFIIA-(beta) and TFIIA-(gamma), and it interacts with both TBP and a TAF (TBP-associated factor). It has been demonstrated that the basic region of TBP is essential for TFIIA-dependent function of TBP.
Catalogue number: BS2282
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug/100ul
Swiss prot: P52655
Dilutions: WB: 1:500~1:1000 IHC: 1:50~1:200
Form: liquid
Storage buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Concentration: 1ug/ul
Antigen: Synthetic peptide, corresponding to amino acids 300-350 of Human TFIIA-(alpha).
Species: TFIIA-(alpha) (E311) pAb detects endogenous levels of TFIIA-(alpha) protein.
Molecular weight: ~ 42 kDa
Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage temp: Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.
Alternative names: General transcription factor II A. 1-like factor; ALF; MGC26254
Additional info: For research use only, not for use in diagnostic procedure.

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