Thrombospondin-1, Recombinant, Mouse, aa19-1171 (THBS1, TSP-1)

ThrombospondinпїЅ1 (TSPпїЅ1) is a 150пїЅ180kD calciumпїЅsensitive protein that is secreted as a disulfideпїЅlinked homotrimer. Within the Thrombospondin family, TSPпїЅ2 is also homotrimeric, while TSPпїЅ3, пїЅ4, and пїЅ5/COMP are pentameric. TSPпїЅ1 regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSPпїЅ1 contains an NпїЅterminal Laminin GпїЅlike globular domain, an extended central region with one vWFпїЅC domain, 3 TSP type 1 repeats, 3 EGFпїЅlike domains, and 8 TSP typeпїЅ3 repeats, and a globular CпїЅterminal lectin domain. Distinct regions of TSPпїЅ1 have been associated with binding to particular ECM or cellular molecules (1, 2). Mature mouse TSPпїЅ1 shares 95% and 99% amino acid sequence identity with human and rat TSPпїЅ1, respectively. TSPпїЅ1 counteracts the angiogenic, hypotensive, and antiпїЅthrombotic effects of nitric oxide (NO) (3). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells (EC), and destabilizes adhesive contacts between EC (4пїЅ6). TSPпїЅ1пїЅs actions on vascular smooth muscle cells support arterial tone and blood pressure in the presence of vasodilatory stimulation (7). It is released from activated platelets and enhances platelet aggregation and adhesion (8). TSPпїЅ1 also plays an important role in wound repair and tissue fibrosis by binding latent TGFпїЅbeta and inducing release of the active cytokine from the latency associated peptide (LAP) (9, 10). TSPпїЅ1 dampens adaptive immune responses by inducing the differentiation of regulatory T cells and inhibiting TCR signaling (11, 12). In the nervous system, it promotes excitatory synapse formation (13, 14) and supports the integration of neural progenitor cells into the olfactory bulb (15). In addition, TSPпїЅ1 is released by apoptotic cells and promotes macrophageпїЅmediated debris clearance (16).\n\nSource:\nRecombinant protein corresponding to aa22-326 from rat Thrombopoietin, NS0-derived.\n\nMolecular Weight:\n~130-155kD\n\nEndotoxin:\n<0.10EU/1ug (LAL method)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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