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TLR4/MD-2 Complex (Toll Like Receptor 4/MD-2) (FITC)

TLR4/MD-2 Complex (Toll Like Receptor 4/MD-2) (FITC)

Cat no: T8050-29C

Supplier: United States Biological
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TLRs are highly conserved throughout evolution and have been implicated in the innate defence to many pathogens. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLRs identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defence to pathogens. TLR4 has been identified next to MD-2 and CD14 as a receptor that is central to the innate immune response to lipopolysaccharides (LPS) of Gram-negative bacteria. TLR4/MD-2 Complex is a TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Catalogue number: T8050-29C
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry, Immunohistochemistry
Size: 100ug
Form: Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography.
Alternative names: TRL4, CD284, lymfocyte antigen 96, ESOP-2
References: 1. Nomura, F et al; Cutting edge: Endotoxin tolerance in mouse peritoneal macrophages correlate with down-regulation of surface toll-like receptor 4 expression. J Immunol 2000, 164: 3471 2. Akashi, S et al; Cutting edge: Cell surface expression and lipopolysaccharide signalling via the toll-like receptor 4-MD-2 complex on mouse peritoneal macrophages. J Immunol 2000, 164: 3471 3. Tapping, RI et al; toll-like receptor 4, but not toll-like receptor 2, is a signalling receptor for Escherichia and Salmonella lipoploysaccharides. J Immunol 2000, 165: 5780 4. Sato, S et al; Synergy and cross-tolerance between toll-like receptor (TLR)2- and TLR4-mediated signalling pathways. J Immunol 2000, 165: 7096 5. Ortega-Cava, C et al; Strategic compartmentalization of toll-like receptor 4 in the mouse gut. J Immunol 2003, 170: 3977
Additional info: Recognizes mouse toll-like receptor 4 (TLR4), when associated with MD-2 and co-precipitates MD-2 with TLR4. The antibody was shown to coprecipitate MD-2 (30kD) with TLR4 (100kD). It does not detect TLR4 alone.

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