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TLR4 (Toll-like Receptor 4, hToll, CD284) (FITC)

TLR4 (Toll-like Receptor 4, hToll, CD284) (FITC)

Cat no: T8050-34N

Supplier: United States Biological
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The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 835aa with approximate molecular weight of 90kD (6). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-2ug/10e6 cells Optimal dilutions to be determined by the researcher. Positive Control: Mouse peritoneal cells Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Catalogue number: T8050-34N
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in 10mM Tris, 150mM sodium chloride, 0.05% BSA, 0.05% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
P type: Mab
Isotype: IgG2a,k
Purity: Purified by Protein G affinity chromatography.
References: 1. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997). 2. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2): 157-161 (2001). 3. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med. 187: 2097-2101 (1998). 4. Takeuchi O., Hoshino K, Kawai T, Sanjo H, Takada H, Ogawa K, Takeda K and Akira S. Immunity 11: 443 (1999). 5. Medzhitov R, Preston-Hurlburt P and Janeway CA Jr. Nature 388 (6640), 394-397 (1997). 6. Poltorak A, Riccardi-Castagnoli P, Citterio S, and Butler B. Proc. Natl. Acad. Sci USA 97: 2163-2167 (2000). 7. Akashi S, Shimazu R, Ogata H, Nagai Y, Takeda K, Kimoto M, Miyake K. J Immunol 164 (7):3471-5 (2000).
Additional info: Recognizes mouse TLR4. Species sequence homology: most mammals.

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