TLR7 (Toll Like Receptor 7, TLR-7, PRO285, UNQ248)

TLR7 is one of toll-like receptors which are type I transmembrane proteins involved in innate immunity by recognizing microbial conserved structures. These proteins are characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain, and act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. Human TLR7(hTLR7) is predominantly expressed in lung, placenta and spleen and hTLR7,8 genes are located on the sex chromosome X. hTLR7 may play an important role in detecting and combating viral infections.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:2000\nOptimal dilutions to be determined by the researcher.\n\nHybridoma:\nDerived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TLR7 protein. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n

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SPECIFICATIONS

Catalog Number

T8050-71M

Size

50ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.02% thimerosal.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Lee J., et al. (2003) Proc. Natl. Acad. Sci. USA. 100, 6646-6651. 2. Crozat K., et al. (2004) Proc. Natl. Acad. Sci. USA. 101, 6835-3836. 3. Muzio M., et al. (1998) J. Exp. Med. 187, 2097-2101. 4. Lund JM., et al. (2002) Nature 420, 329-333.

Additional Info

Recognizes human TLR7, 16kD.