TLR7 (Toll Like Receptor 7, TLR-7, PRO285, UNQ248)

Toll Like Receptors (TLRs) are the mammalian homologues of the Drosophila Toll protein, which is a transmembrane receptor. TLR2 and TLR4 are essential components for recognition of distinct bacterial cell wall components. TLRs have been shown to participate in the recognition of pathogens by the innate immune system. The cytoplasmic domain of TLR2 can form functional pairs with TLR6 or TLR1. This interaction leads to cytokine induction. TLR6, TLR2 and TLR1 are recruited to macrophage phagosomes that contain IgG-coated erythrocytes that do not display microbial components. TLRs sample the contents of the phagosome independent of the nature of the contents. This sampling can establish a combinatorial repertoire to discriminate among the large number of pathogen-associated molecular patterns found in nature. Bacterial components elicit the activation of an intracellular signaling cascade via TLR in a way similar to that which occurs upon ligand binding to the IL-1 receptor. This signaling pathway leads to the activation of a transcription factor NF-B and c-Jun N-terminal kinase (JNK) and initiates the transcription of pro-inflammatory cytokine genes. TLRs and the downstream signaling pathway play a key role in innate immune recognition and in the subsequent activation of adaptive immunity.\n\nApplications: \nSuitable for use in ELISA, Western Blot Not suitable for use in Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1.0ug/ml (Direct)\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nAntibody Binding Inhibition: \n~25x ug/ml per ug/ml antibody.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8050-75B

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before teh addition of glycerol to 40%.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Visitin, A., et al., J. Immunol. 166: 249-255 (2001). 2. Akira, S., et al., Immunol. Lett. 85: 85-95 (2003). 3. Pinhal-Enfield, G., et al., Am. J. Pathol. 163: 711-721 (2003). 4. Ito, T., et al., Hum. Immunol. 63: 1120-1125 (2002). 5. Okada, T., et al., Eur. J. Immunol. 33: 1012-1019 (2003). 6. Rothenfusser, S., et al., Hum. Immunol. 63: 1111-1119 (2002). 7. Takeuchi, O., et al., Int. Immunopharmacol. 1: 625-635 (2001). 8. Ozinsky, A., et al., PNAS 97: 13,766-13,771 (2000). 9. Xu, Y., et al., Nature 408: 111-115 (2000). 4. Chuang, T.H., Ulevitch, R.J., Eur. Cytokine Netw. 11: 372-378 (2000). 10. Du, X., et al., Eur. Cytokine Netw. 11: 362-371 (2000).

Additional Info

Reacts with human TLR7 protein.By Western Blot, identifies a single band at ~110kD. Reactivity has been confirmed with human placenta tissue homogenates, JEG-3 placenta choriocarcinoma, BC-1 human Blymphocyte lymphoma, Nalm-6 pre-B lymphocyte and REH pre-B lymphocyte cell lysates. Species crossreactivity: Expected with mouse TLR7 based on sequence homology.