Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infections and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88, and TIRAP to activate various kinases and transcription factors. Like TLR7, TLR8 is localized to endosomal or lysosomal compartments and stimulates the innate immune response after activation by guanosine- and uridine-rich single-stranded RNA. Human, but not murine TLR8, confers responsiveness to the antiviral compound R848.
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution:
ELISA: 1:2000-1:10,000
Western Blot: 1:500-1:2000
Immunohistochemistry (Paraffin): 5ug/ml
Optimal dilutions to be determined by researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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