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TLR9 (Toll Like Receptor 9, Toll-like Receptor 9, Toll-like Receptor 9 Isoform A Precursor, Toll-like Receptor 9 Isoform B, TLR 9, TLR-9, CD289)

TLR9 (Toll Like Receptor 9, Toll-like Receptor 9, Toll-like Receptor 9 Isoform A Precursor, Toll-like Receptor 9 Isoform B, TLR 9, TLR-9, CD289)

Cat no: T8050-90M

Supplier: United States Biological
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TLR9 is a member of the Toll-like receptor (TLR) family and functions as a receptor for bacterial DNA (CpG-DNA), playing a fundamental role in pathogen recognition and activation of innate immunity. TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents.They also initiate cell signalling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul Western Blot: 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: T8050-90M
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Western Blot
Size: 250ug
Form: Supplied as a liquid, 0.09% sodium azide.
P type: Mab
Isotype: IgG2a
Purity: Purified
Additional info: Recognizes human TLR9. Species Crossreactivity: canine, murine. In mouse RAW264 cells TLR9 is expressed intracellularly, but not at the cell surface.

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