TMED10, ID (Transmembrane emp24 Domain-containing Protein 10, 21kD Transmembrane-trafficking Protein, S31III125, S31I125, Tmp-21-I, Transmembrane Protein Tmp21, p23, p24 Family Protein delta-1, p24delta, TMP21)

TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the y-secretase but not the esecretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the y and e-sites and can lead to the accumulation of the Ab cleavage product that is associated with Alzheimer's disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased y-secretase activity but not e-secretase activity, and increased Ab production, demonstrating that TMP21 can modulate ysecretase activity.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMouse Brain Tissue Lysate or Human Brain Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146144

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Blunt R, Feick P, Puype M, et al. Tmp21 and p24A, two type I proteins enriched in pancreatic microsomal membranes, are members of a protein family involved in vesicular trafficking. J. Biol. Chem. 1996; 271:17183-9. 2. Chen F, Hasegawa H, Schmitt-Ulms G, et al. TMP21 is a presenilin complex component that modulates ?-secretase but not y-secretase activity. Nature 2006; 440:1208-12. 3. Periz G and Fortini ME. Functional reconstitution of secretase through coordinated expression of presenilin, nicastrin, aph-1, and pen-2. J. Neurosci. Res. 2004; 77:309-22. 4. Selkoe DJ. The cell biology of-amyloid precursor protein and presenilin in Alzheimer's disease. Trends Cell Biol. 1998; 8:447-53.

Additional Info

Recognizes human TMP21. Species Crossreactivity: mouse and rat.