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TNF-a, ID (Tumor Necrosis Factor a (TNF Superfamily, Member 2), Tnfa)

TNF-a, ID (Tumor Necrosis Factor a (TNF Superfamily, Member 2), Tnfa)

Cat no: T9160-37F

Supplier: United States Biological
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Tumor necrosis factor alpha (TNF- a) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. The primary role of TNF is in the regulation of immune cells. TNF is also able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. TNF-a was also wildly used a a marker in zebrafish (Danio rerio) models in the studies of immune responds to the infections and other pathogenesis degeneration, and apoptosis. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:5000-1:20,000 Western Blot: 1:500-1:1000 Immunohistochemistry (Cryosection-PFA fixed): 1:50-1:200 Optimal dilutions to be determined by the researcher. Control Peptide: T9160-37P Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: T9160-37F
Reactivities: Zebrafish/Fish
Hosts: Rabbit
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 250ul
Form: Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Vojtech, L.N., et al. (2009) Infect. Immun. 77(2):914-925. 2. Matthews, R.P., et al. (2009) Development 136(5):865-875. 3. Rodruez, I., et al. (2008) Fish Shellfish Immunol. 25(3):239-249. 4. Watzke, J., et al. (2007) Fish Shellfish Immunol. 23(4):901-905. 5. Clay, Het al. (2007) Cell Host Microbe. 2(1):29-39. 6. Nayak, A.S., et al. (2007) Toxicol. Sci. 98(1):118-124. 7. Levraud, J.P., et al. (2007) J. Immunol. 178(7):4385-4394. 8. Rojo, I., et al. (2007) Fish Shellfish Immunol. 23(6):1285-1293. 9. Eimon, P.M., et al. (2006) Cell Death Differ. 13(10):1619-1630.
Additional info: Recognizes zebrafish TNF-a.

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