TNF-a, ID (Tumor Necrosis Factor a (TNF Superfamily, Member 2), Tnfa)

Tumor necrosis factor alpha (TNF- a) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. The primary role of TNF is in the regulation of immune cells. TNF is also able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. TNF-a was also wildly used a a marker in zebrafish (Danio rerio) models in the studies of immune responds to the infections and other pathogenesis degeneration, and apoptosis.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:5000-1:20,000\nWestern Blot: 1:500-1:1000\nImmunohistochemistry (Cryosection-PFA fixed): 1:50-1:200\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: T9160-37P\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T9160-37F

Size

250ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

ZF/Fish

Form

Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Vojtech, L.N., et al. (2009) Infect. Immun. 77(2):914-925. 2. Matthews, R.P., et al. (2009) Development 136(5):865-875. 3. Rodruez, I., et al. (2008) Fish Shellfish Immunol. 25(3):239-249. 4. Watzke, J., et al. (2007) Fish Shellfish Immunol. 23(4):901-905. 5. Clay, Het al. (2007) Cell Host Microbe. 2(1):29-39. 6. Nayak, A.S., et al. (2007) Toxicol. Sci. 98(1):118-124. 7. Levraud, J.P., et al. (2007) J. Immunol. 178(7):4385-4394. 8. Rojo, I., et al. (2007) Fish Shellfish Immunol. 23(6):1285-1293. 9. Eimon, P.M., et al. (2006) Cell Death Differ. 13(10):1619-1630.

Additional Info

Recognizes zebrafish TNF-a.