Trypanosoma cruzi, the protozoan parasite that causes Chagas' disease in humans, has a complex life cycle alternating between the insect vector and the mammalian host. In the vector, it multiplies as noninfective epimastigotes that migrate to the hindgut and differentiate into infective metacyclic trypomastigotes. A lipophosphoglycan (LPG)-like glycoconjugate is expressed on the cell surface of Trypanosoma cruzi epimastigotes. It migrates as a homogeneous band (42kD). T. cruzi LPG has large amounts of glucosamine and sialic acid as well as galactosamine and is anchored on the cell surface via an alkylacylphosphatidylinositol.
Applications:
Suitable for use in ELISA, Western Blot, and Immunofluorescence. Other applications not tested.
Recommended Dilutions:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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