Tubulin, alpha, NT (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

The alpha-tubulin (relative molecular weight about 50kD) is globular protein that exists in\ncells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In\ndifferent species it is coded by multiple tubulin genes that form tubulin classes (in human 6\ngenes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes\nare expressed ubiquitously, while some have more restricted tissue expression.\nAlpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes\nand their posttranslational modifications are responsible for multiple tubulin charge variants -\ntubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural\ndomain.The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and\nhave an outer diameter 25nm. Microtubules have their intrinsic polarity; highly dynamic plus\nends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains.\n\nApplications: \nSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2mg/ml under reducing conditions. Prepare samples in Laemmli reducing buffer.\nImmunocytochemistry: Use fixed and permeabilized cells.\nOptimal dilutions to be determined by the researcher.\n\nWB Positive Control: \nHPB-ALL peripheral blood leukemia cell lysate (incubation 60 min)\nPorcine brain lysate (incubation 90 min)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T9154-01J

Size

100ug

Applications

ICC, WB

Hosts

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

P Type

Mab

Purity

Purified from ascites by precipitation methods.

Isotype

IgG1

References

1. Viklicky V, Draber P, Hasek J, Bartek J.: Production and characterization of a monoclonal antitubulin antibody. Cell Biol Int Rep. 1982 Aug;6(8):725-31. 2. Grimm M, Breitling F, Little M.: Location of the epitope for the alpha-tubulin monoclonal antibody TU-O1. Biochim Biophys Acta. 1987 Jul 24;914(1):83-8. 3. Linhartova I, Draber P, Draberova E, Viklicky V.: Immunological discrimination of\nbeta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins. Biochem J. 1992 Dec 15;288 ( Pt 3):919-24. 4. Draber P, Draberova E, Zicconi D, Sellitto C, Viklicky V, Cappuccinelli P.: Heterogeneity of microtubules recognized by monoclonal antibodies to alpha-tubulin. Eur J Cell Biol. 1986 Jun;41(1):82-8. 5. Draber P, Draberova E, Linhartova I, Viklicky V.: Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies. J Cell Sci. 1989 Mar;92 ( Pt 3):519-28. 6. Draber P, Draberova E, Viklicky V.: Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta tubulin epitope in the sperm head. Histochemistry. \n1991;95 (5):519-24. 7. Smertenko A, Blume Y, Viklicky V, Opatrny Z, Draber P.: Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells. Planta. 1997;201(3):349-58.

Additional Info

Recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha tubulin. \nSpecies Crossreactivity: All (recognized epitope conserved within all species).