TWIST1 pAb

Members of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes. Class A proteins include the ubiquitously expressed E-box binding factors E12/E47, ITF2 and HEB (BETA1 or HTF4). Class B proteins such as MyoD, myogenin and NeuroD (BETA2) are transiently expressed and exhibit a much more limited tissue distribution. Class A proteins heterodimerize with class B proteins to activate DNA transcription. Working in opposition to these positively acting factors are a specialized group of proteins that function as dominant negative regulators. Muscle tissue is derived from a subset of cells originating from the embryonic mesoderm. The novel basic helix-loop-helix (bHLH) transcription factor twist is a putative regulator of mesodermal differentiation and myogenesis. Twist is expressed throughout the epithelial somite but not in the myotome. Twist requires dimerization with the E proteins and inhibits myogenic regulatory factors. It has been implicated as regulator of the temporal and spatial formation of myotomes.

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SPECIFICATIONS

Catalog Number

BS60412

Size

100ug/100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

A synthetic peptide corresponding to residues in Human TWIST1.

Species

TWIST1 pAb detects endogenous levels of TWIST1 protein.

Dilutions

WB: 1:500~1:1000\r

Swiss Prot

Q15672

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul