UbcH7, Recombinant, Human (Ubiquitin-conjugating enzyme E2 L3, UBE2L3, Ubiquitin-conjugating enzyme E2-F1, L-UBC, Ubiquitin carrier protein L3, Ubiquitin protein ligase L3)

Human Ubquitin-conjugating enzyme 7 (UbcH7) is a ubiquitin-conjugating enzyme (E2) mediating c-fos degradation, transcription factor NF-kB maturation, and human papilloma virus-mediated p53 and Myc protein degradation, in vitro. The ubiquitin-conjugating enzymes (E2s) are essential components of the post-translational protein ubiquitination pathway, mediating the transfer of activated ubiquitin to substrate proteins. The human UBE2L1-UBE2L4 gene could potentially encode different isoforms of the UbcH7. UBE2L3 gene, located at chromosome 22q11.2, is the only identical family member with introns and encodes a polypeptide sequence identical to that of UbcH7 (NP_003338).\n\nMolecular Weight: \n17.9kD (154 amino acids). Does not contain His tag.\n\nAA Sequence:\nMAASRRLMKE LEEIRKCGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA FRIEINFPAE\nYPFKPPKITF KTKIYHPNID EKGQVCLPVI SAENWKPATK TDQVIQSLIA LVNDPQPEHP\nLRADLAEEYS KDRKKFCKNA EEFTKKYGEK RPVD\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U0880-02

Size

250ug

Form

Supplied as a liquid in 50mM HEPES, pH 7.5, 150mM sodium chloride, 1mM DTT and 10% glycerol.

Purity

~95% (SDS PAGE) Purified by conventional chromatography techniques.

References

1. Ardley, H.C., et al., (2000) Biochim. Biophys. Acta. 1491(1-3):57-64. 2. Moynihan, T.P., et al., (1998) Genomics 51(1):124-127. 3. Nuber, U., et al., (1996) J. Biol. Chem. 271(5):2795-2800.