Kindlin-3 is an intracellular protein encoded by the FERMT3 gene and which binds directly to the beta chain cytoplasmic tail of integrin in hematopoietic cells. This protein belongs to a family of adhesion plaque proteins, of which there are three members, which act to activate integrins. Kindlin-3 differs from Kindlins-1 and -2 in that its expression is restricted to hematopoietic systems and is especially high in spleen and megakaryocytes. It is a necessary component for integrin activation and for platelet aggregation. Mutations in FERMT3 and consequently to the encoding of Kindlin-3 protein are causal to LAD-I variant syndrome, an uncommon genetic disorder in which integrin adhesive functions on platelets and leukocytes are disrupted.
Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 0.5ug/ml
Immunocytochemistry: 1:500
Optimal dilutions to be determined by the researcher.
Positive Control:
Human lymph node tissue lysate
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.