Urokinase Plasminogen Activator Receptor, Human ELISA Kit, BioAssay(TM) (uPAR)

Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Urokinase-type Plasminogen Activator Receptor (uPAR) in cell culture supernates and serum. Each kit contains sufficient materials to run ELISAs on approximately fifteen 96-well plates.\n\nKit Components:\nU2610-06A: Capture Antibody 1x1 vial uPAR Pab MoxHu Concentration, ~700ug/ml, Supplied as a lyophilized powder. Reconstitute with 1ml sterile sterile PBS. Working dilution; 4ug/ml in PBS.\nU2610-06B: Detection Antibody (Biotin) 1x1 vial Concentration, ~36ug/ml Supplied as a lyophilized powder. Reconstitute with 1ml sterile Reagent Diluent (Not supplied). Working dilution; 200ng/ml in Reagent Diluent.\n*U2610-06C: Standard 3x1 vial Concentration, ~160ng/ml Supplied as a lyophilized powder. Reconstitute with 500ul of sterile ddH2O. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. Aliquot and store at -70 degrees C for up to 6 months. A seven point standard curve using 2-fold serial dilutions in Reagent Diluent, and a high standard of 2000pg/ml is recommended. \nU2610-06D: Streptavidin (HRP) 1x1ml Store at 4 degrees C for up to 6 months. DO NOT FREEZE. Dilute 1/200 using Reagent Diluent.\n\nStorage and Stability:\nStore *U2610-06C powder at 4 degrees C, liquid at -70 degrees C. Store U2610-06A and U2610-06B powder at 4 degrees C, liquid at -20 degrees C. Store U2610-06D at 4 degrees C. DO NOT FREEZE. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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