USP10, Active, Recombinant, Human, FLAG-Tag (Ubiquitin Carboxyl-terminal Hydrolase 10, Deubiquitinating Enzyme 10, Ubiquitin Thiolesterase 10, Ubiquitin-specific-processing Protease 10, KIAA0190)

USP10 is a hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. It acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, USP10 translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. It is a component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. USP10 does not deubiquitinate MDM2. It deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling.\n\nSource:\nRecombinant corresponding to aa2-798 from human USP10, fused to FLAG-tag at N-terminal, expressedin Sf9 insect cells via a baculovirus expression system.\n\nMolecular Weight:\n~88kD\n\nApplications: \nSuitable for for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137394

Size

50ug

Form

Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM sodium chloride, 2.4mM potassium chloride, 90ug/ml FLAG peptide, 10% glycerol, 3mM DTT.

Purity

Purified (~60%)

References

1. Yuan, J., et al. (2010). Cell 140(3): 384-96. 2. Soncini, C., et al. (2001). Oncogene 20(29):3869-79. 3. Faus, H., et al. (2005). Mol. Cell. Endocrinol. 245(1-2):138-46. 4. Boulkroun, S., et al., Am. J. Physiol. 295: F889-F900 (2008). 5. Bomberger, J.M., et al., J. Biol. Chem. 284: 18,778-18,789 (2009). 6. Liu, J., et al., Cell 147: 223-234 (2011).