Home  >  Products  >  USP10 (Ubiquitin Specific Peptidase 10, Ubiquitin Specific-processing Protease 10, Deubiquitinating Enzyme 10, KIAA0190, MGC2621, Ubiquitin Carboxyl Terminal Hydrolase 10, Ubiquitin Thioesterase 10, UBPO)
USP10 (Ubiquitin Specific Peptidase 10, Ubiquitin Specific-processing Protease 10, Deubiquitinating Enzyme 10, KIAA0190, MGC2621, Ubiquitin Carboxyl Terminal Hydrolase 10, Ubiquitin Thioesterase 10, UBPO)

USP10 (Ubiquitin Specific Peptidase 10, Ubiquitin Specific-processing Protease 10, Deubiquitinating Enzyme 10, KIAA0190, MGC2621, Ubiquitin Carboxyl Terminal Hydrolase 10, Ubiquitin Thioesterase 10, UBPO)

Cat no: U4100-15D

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. USP10 possessesaa sequences that match the consensus cysteine and histidine boxes representative of the USP family of deubiquitinating enzymes. At the posttranslational level, USP10 appears to be regulated through both protein-protein interactions and phosphorylation. Indeed, interaction of USP10 with Ras-GAP SH3 domain binding protein (G3BP) has been found to inhibit its ability to catalyze the disassembly of ubiquitin chains (3). Furthermore, ATM-mediated phosphorylation of USP10 at Thr42 and Ser337 was shown to promote its stabilization and redistribution from the cytoplasm to the nucleus, where it functions in p53 deubiquitination, stabilization, and activation in response to genotoxic stress (4). USP10 also functions in the endosomal compartment, where it has been shown to deubiquitinate CFTR in order to enhance its endocytic recycling and cell surface expression (5,6). Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:50 Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: U4100-15D
Reactivities: Human, Mouse, Rat, Non-Human Primate
Hosts: Rabbit
Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: (1) Nijman, S.M. et al. (2005) Cell 123, 773-86. (2) Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613. (3) Soncini, C. et al. (2001) Oncogene 20, 3869-79. (4) Yuan, J. et al. (2010) Cell 140, 384-96. (5) Bomberger, J.M. et al. (2009) J Biol Chem 284, 18778-89. (6) Bomberger, J.M. et al. (2010) Channels (Austin) 4.
Additional info: Recognizes endogenous levels of total USP10. Species Crossreactivity: monkey, mouse, rat.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave