USP7 (Ubiquitin Carboxyl-terminal Hydrolase 7, Ubiquitin Thiolesterase 7, Ubiquitin-specific processing Protease 7, Deubiquitinating Enzyme 7, Herpes Virus-associated Ubiquitin-specific Protease, HAUSP)

USP7 (ubiquitin specific peptidase 7) is a nuclear protein belonging to the peptidase C19 family with a MATH domain and is known to cleave ubiquitin fusion protein substrates. It is a monomer that interacts with proteins like TP53, MDM2 and UBXN6 and also with Herpes virus 1 trans-acting transcriptional protein ICP0/VMW110 and Epstein-Barr virus EBNA1 and this binding may modulate the substrate specificity or activity of USP7 to stabilize viral proteins. USP7 deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2, and is known to induce TP53-dependent cell growth repression and apoptosis. USP7 is generally inhibited by N-ethyl-maleimide (NEM) and divalent cations. Though it tolerates high concentrations of NaCl, it is inhibited at concentrations of 195 mM and higher. USP7 is widely expressed in most of the tissues and might be present in a minority of ND10 nuclear bodies.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMCF7 cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

U4100-01A

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can, Ch/Bird, Moll

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Li, M. et al. Nature. 416:648-653 (2002). 2. Holowaty, MN. et al. J. Biol. Chem. 278:47753-47761 (2003). 3. Song, MS. et al. Nature. 455:813-817 (2008).

Additional Info

Recognizes human USP7. Species Crossreactivity: chicken, chimpanzee, canine, equine, human, macaque, mouse, rat Species sequence homology: pufferfish.