Home  >  Products  >  USP7 (Ubiquitin Carboxyl-terminal Hydrolase 7, Ubiquitin Thiolesterase 7, Ubiquitin-specific processing Protease 7, Deubiquitinating Enzyme 7, Herpes Virus-associated Ubiquitin-specific Protease, HAUSP)
USP7 (Ubiquitin Carboxyl-terminal Hydrolase 7, Ubiquitin Thiolesterase 7, Ubiquitin-specific processing Protease 7, Deubiquitinating Enzyme 7, Herpes Virus-associated Ubiquitin-specific Protease, HAUSP)

USP7 (Ubiquitin Carboxyl-terminal Hydrolase 7, Ubiquitin Thiolesterase 7, Ubiquitin-specific processing Protease 7, Deubiquitinating Enzyme 7, Herpes Virus-associated Ubiquitin-specific Protease, HAUSP)

Cat no: U4100-01B

Supplier: United States Biological
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USP7 (ubiquitin specific peptidase 7) is a nuclear protein belonging to the peptidase C19 family with a MATH domain and is known to cleave ubiquitin fusion protein substrates. It is a monomer that interacts with proteins like TP53, MDM2 and UBXN6 and also with Herpes virus 1 trans-acting transcriptional protein ICP0/VMW110 and Epstein-Barr virus EBNA1 and this binding may modulate the substrate specificity or activity of USP7 to stabilize viral proteins. USP7 deubiquitinates TP53/p53 and MDM2 and strongly stabilizes TP53 even in the presence of excess MDM2, and is known to induce TP53-dependent cell growth repression and apoptosis. USP7 is generally inhibited by N-ethyl-maleimide (NEM) and divalent cations. Though it tolerates high concentrations of NaCl, it is inhibited at concentrations of 195 mM and higher. USP7 is widely expressed in most of the tissues and might be present in a minority of ND10 nuclear bodies. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: MCF7 cell lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: U4100-01B
Reactivities: Human, Mouse, Rat, Canine, Chicken/Bird, Mollusc
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: 1. Li, M. et al. Nature. 416:648-653 (2002). 2. Holowaty, MN. et al. J. Biol. Chem. 278:47753-47761 (2003). 3. Song, MS. et al. Nature. 455:813-817 (2008).
Additional info: Recognizes human USP7. Species Crossreactivity: chicken, chimpanzee, canine, equine, macaque, mouse, rat. Species sequence homology: pufferfish.

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