USP7 (Ubiquitin specific peptidase 7 (herpes virus-associated), Deubiquitinating enzyme 7, HAUSP, Herpes virus-associated ubiquitin-specific protease, TEF1, Ubiquitin carboxyl-terminal hydrolase 7, Ubiquitin-specific-processing protease 7, Ubiquitin thioe

USP7 (Ubiquitin-specific-processing protease 7) is a deubiquitinase that has been shown to regulate the p53-Mdm2 pathway (1). Cotransfection of p53 and USP7 stabilizes p53 through the removal of ubiquitin moieties from polyubiquitinated p53 (1). It also counteracts the destabilizing effect of Mdm2 by direct deubiquitination of p53 (2). Subsequently, USP7 has been shown to deubiquitinate Mdm2 and Mdmx, thereby stabilizing these proteins (2). USP7 interacts with Herpes virus 1 trans-acting transcriptional protein ICP0/VMW110 and Epstein-Barr virus EBNA1. EBNA1 shows a tenfold higher affinity than TP53 and can compete with it for USP7 binding (3).\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.\nOther applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:10,000\nImmunohistochemistry (Paraffin): 1:50-1:100\nImmunocytochemistry: 1:100-1:250\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

U4100-02A

Size

100ul

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Kon N et al. Oncogene. 29(9):1270-9, 2010, 2. Meulmeester E et al. Cell Cycle. 4(9):1166-70, 2005, 3. The UniProt Consortium, The Universal Protein Resource (UniProt) in 2010, Nucleic Acids Res. D142-D148 (2010).

Additional Info

Recognizes human USP7 at 135kD. Species Crossreactivity: mouse, rat.