Home  >  Products  >  Vacuolar Protein Sorting 28 (Yeast) (VVPS 28, VPS28, MGC60323, Tumor Susceptibility Gene 101 TSG101, Yeast Class E Protein Vps28p Homolog)
Vacuolar Protein Sorting 28 (Yeast) (VVPS 28, VPS28, MGC60323, Tumor Susceptibility Gene 101 TSG101, Yeast Class E Protein Vps28p Homolog)

Vacuolar Protein Sorting 28 (Yeast) (VVPS 28, VPS28, MGC60323, Tumor Susceptibility Gene 101 TSG101, Yeast Class E Protein Vps28p Homolog)

Cat no: V0650-02

Supplier: United States Biological
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This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Positive Control: Human testis and brain lysates. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: V0650-02
Reactivities: Human, Mouse, Xenopus/Amphibian, Zebrafish/Fish
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: Bishop N & Woodman P TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes. J Biol Chem 276:11735-42 (2001).
Additional info: Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse (100% identity with immunogen), Xenopus laevis (100% identity with immunogen) and Zebrafish (100% identity with immunogen) due to sequence homology.

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