VAMP 2 (Vesicle-Associated Membrane Protein 2, Synaptobrevin 2)

VAMP-2 (vesicle associated membrane protein 2; also synaptobrevin-2) is a 13kD member of the synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is almost completely transmembrane) that is found in the presynaptic terminals of neurons. VAMP-2 is targeted to presynaptic vesicles following binding to synaptophysin I. Dissociation allows for synaptic vesicle fusion at the synaptic cleft with subsequent granule release. Human VAMP-2 is 116aa in length. It contains one acetylation site at Ser2, a vSNARE coiled-coil homology region (aa31-91), and a membrane-anchor domain (aa95-114).\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000; Rat brain membranes\nImmunohistochemistry: Mouse brain sections\nOptimal dilutions determined by the researcher.\n\nControl Antigen Included: \nV2024-02AA\n\nPreadsorption Control: \n3ug fusion protein per 1ug antibody\n\nPositive Control in Western Blot: \n20ng fusion protein/Minigel lane\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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