VAMP 2 (Vesicle Associated Membrane Protein 2, Vesicle-associated Membrane Protein 2, VAMP2, VAMP-2, Synaptobrevin 2, Synaptobrevin-2, SYB 2, SYB2, SYB-2)

Cat no: V2024-02N

Supplier: United States Biological

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Additional Information

Vesicle-associated membrane protein (VAMP), also known as synaptobrevin, is an 18kD integral membrane protein localized to the cytoplasmic surface of synaptic vesicle (2). VAMP consists of a proline-rich amino-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a carboxylterminal tail (3). Two VAMP homologs, VAMP1 and VAMP2, are differentially expressed in the nervous system (3). VAMP1 expression is localized to neurons involved in modulating overlapping patterns, whereas VAMP2 is found in neurons ssociated with autonomic, sensory and integrative functions. In non-neuronal tissues, VAMP1 is restricted to pancreas and kidney tubular cells, whereas VAMP2 is predominantly expressed in Langerhans islets and glomerular cells (4). Evidences for VAMP Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Other applications not tested. Recommend Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:10-1:50 Immunofluorescence (IC): 1:10-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	V2024-02N
Reactivities:	Human, Mouse, Rat, Bovine, Guinea Pig, Rabbit
Hosts:	Rabbit
Applications:	Immunofluorescence, Immunoprecipitation, Western Blot
Size:	50ug
Form:	Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
P type:	Pab
Isotype:	IgG
Purity:	Purified by Protein A affinity chromatography.
References:	1. Pevsner, J., Hsu, S. C., Braun, J. E. A., Calakos, N., Ting, A. E., Bennett, M. K., Scheller, R. H. (1994) Neuron 13: 353-361.2. Calakos, N., and Scheller, R. (1996) Physiological Reviews 76: 1-29. 3. Trimble, W.S., Gray, R.S., Wlferink, L.A., Wilson, M.C. and Scheller, R.H. (1990) J. Neurosci. 10: 1380-1387. 4. Rossetto, O., Gorza, L., Schiavo, G., Schiavo, N., Scheller, R.H. and Montecucco, C. (1996) J. Cell Biology 132: 167-179. 5. Protopopov, V., Govindan, B., Novick, P. and Gerst, J.E. (1993) Cell 74: 855-861. 6. Schiavo G., Benfenati, F., Poulain, B., Rossetto, O., Polverino de Laureto, P., Dasgupta, B.R., and Montecucco, C. (1992) Nature 359: 832-835. 7. Sollner, T., Bennett, M.K., Whiteheart, S.W., Scheller, R.H., Rothaman, J.E. (1993) Cell 75: 409-418. 8. Mott, J., Barnewall, R.E., Rikihisa, Y. (1999) Infection and Immunity 67: 1368-1378. 9. Daly, C., Ziff, E.B. (2002) J Biol Chem 277(11): 9010-9015. 10. Ohara-Imaizumi, M., Nakamichi, Y., Nishiwaki, C., Nagamatsu, S. (2002) J Biol Chem 277(52): 50805-50811. 11. Wang, Y., Jerdeva, G., Yarber, F.A., Da Costa, S.R., Xie, J., Qian, L., Rose, C.M., Mazurek, C., Kasahara, N., Mircheff, A.K., Hamm-Alvarez, S.F. (2003) J Cell Sci 116(10): 2051-2065
Additional info:	Recognizes rat VAMP 2 at ~18kD. Species Crossreactivity: human, mouse, bovine, guinea pig, rabbit

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VAMP 2 (Vesicle Associated Membrane Protein 2, Vesicle-associated Membrane Protein 2, VAMP2, VAMP-2, Synaptobrevin 2, Synaptobrevin-2, SYB 2, SYB2, SYB-2)

Cat no: V2024-02N

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