VAMP 3 (Vesicle Associated Membrane Protein 3, Vesicle-associated Membrane Protein 3, VAMP3, VAMP-3, Cellubrevin, CEB, Synaptobrevin 3, Synaptobrevin-3, SYB3)

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25kD (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer?s disease, all VAMP isoform levels appear to be significantly reduced.\n\nApplications: \nSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2024-55A

Size

100ug

Applications

IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can

Form

PBS with 1mg/ml BSA containing 0.05% Sodium azide

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Annaert WG et al. Export of cellubrevin from the endoplasmic reticulum is controlled by BAP31. J Cell Biol 139:1397-410 (1997). <PUBMED:9396746>McMahon HT et al. Cellubrevin is a ubiquitous tetanus-toxin substrate homologous to a putative synaptic vesicle fusion protein. Nature 364:346-9 (1993). <PUBMED:8332193>

Additional Info

Species Crossreactivity: Crossreacts with canine, Human, Mouse and Rat.