Vascular Endothelial Growth Factor, Mature (VEGF-C, VEGF-A, Vascular permeability factor, VPF)

Vascular endothelial growth factors (VEGFs), a group of structurally and functionally related growth factors, may play important roles in vascular system formation during embryonic development, the regulation of capillary growth in adults, and the maintenance of normal vasculature. VEGF-C is a ligand for the receptor tyrosine kinases VEGFR-3 and VEGFR, bothof which are expressed by endothelial cells. VEGF-C mRNA has been detected in human heart, placenta, muscle, ovary, and small intestine, as well as in malignant tissues. A study of 35 tumor samples detected VEGF-C mRNA expression in over 50% of tumor specimens, including breast carcinomas, squamous cell carcinomas, melanomas, sarcomas, and adenomas, with notable low-level consistency for all lymphomas examined. VEGF-C has been implicated in lymphangiogenesis, physiological angiogenesis, and the development and progression of angiogenic diseases, including the angiogenesis associated with breast cancers. \n\nApplications:\nSuitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2110-04C

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Li X, et al. Int J Biochem Cell Biol 33(4):421-426, 2001. 2. Partanen TA, et al. FASEB J 14(13):2087-2096, 2000. 3. Salven P, et al. Am J Pathol 153(1):103-108, 1998. 4. Cao Y, et al. PNAS 95:14389-14394, 1998. 5. Valtola R, et al. Am J Pathol 154(5):1381-1390, 1999. 6. Olofsson B, et al. Curr Opin Biotechnol 10:528-535, 1999. 7. Joukov V, et al. EMBO J 16(13):3898-3911, 1997.

Additional Info

Recognizes the mature form of human VEGF-C. No cross-reactivity with other species has been detected. On Western blots, this antibody recognizes the mature form of VEGF-C at 21kD, as well as forms of VEGF-C at other points in its proteolytic processing (43 and 58/61kD). Reactivity is confirmed with human PC-3 prostate adenocarcinoma cell lysates.